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KMID : 0525720080130010022
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Journal of Chitin and Chitosan
2008 Volume.13 No. 1 p.22 ~ p.29
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Preparation of Cisplatin-Loaded Carboxymethyl Chitosan Nanoparticles
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Nam Joung-Pyo
Kim Dong-Gon
Jeong Young-Il
Kim Myung-Yul
Jang Mi-Kyeong
Nah Jae-Woon
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Abstract
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Carboxymethyl chitosan(CMCh) was prepared by introduction of caroxymethyl group to C6-OH (O-carboxymethyl chitosan, OCMCh) or C2-NH2 (N-carboxymethylc chitosan, NCMCh) position of low molecular weight water soluble chitosan (LMWSC). Composition of CMCh was confirmed by proton-nuclear magnetic resonance spectroscopy (1 H NMR). Cisplatinincorporated nanoparticles were prepared by simple mixing of cisplatin and CMCh. Nanoparticles can be prepared by ion complex formation between cisplatin and CMCh. When the feeding amount of cisplatin was increased, drug contents was increased and loading efficiency was decreased. Furthermore, particle size became larger size and zeta potential was decreased by increasing the feeding amount of drug. At the observation of transmission electron microscopy (TEM), cisplatin-incorporated CMCh nanoparticles have spherical shapes with diameter of about 100 nm ~ 300 nm. At drug release study, cisplatin release rate was decreased by increased feeding amount of cisplatin. Nanoparticles from OCMCh showed faster cisplatin release properties than that of NCMCh. Cell cytotoxicity of cisplatin-incorporated CMCh nanoparticles were tested with human embryonic kidney cell, 293T. In the case of cisplatin itself, survived cells were gradually decreased by increasing the concentration of cisplatin from 0.01 μg/ml to 100 μg/ml. When nanoparticles were treated, however, higher than 80% of cells were survived at 10 μg/ml equivalent concentration of cisplatin and about 70% of cells were survived at 100 μg/ml. These results showed that cisplatinincorporated CMCh nanoparticles can be considered as a predominent cisplatin delivery carriers.
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KEYWORD
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Carboxymethyl chitosan, LMWSC, Cisplatin, Nanoparticle, Ion complex
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